Journal: Scientific Reports

Article Title: Revisiting bioluminescence and sucrose utilization in aquatic pathogens Vibrio harveyi and V. campbellii using genome-wide in silico mapping and phenotyping

doi: 10.1038/s41598-026-37651-3

Figure Lengend Snippet: Maximum likelihood Phylogenetic analysis of concatenated sequence of luminescence (LuxCDABEG) ( A ) and ScrRAKB ( B ) in V. harveyi and V. campbellii. The strains having full set of LuxCDABEG and ScrRAKB were selected for phyologenetic analysis. V. vulnificus ATCC 43382 served as outgroup for bioluminescence and V. alginolyticus ATCC 17749 for ScrRAKB clustering. Strains sequenced in the present study has been shown in blue color. Bootstrap value of more than 0.75 has been shown as star. Increasing size of star indicates higher bootstrap value. The tree was visualized and annotated using iTOL. Vh, V. harveyi ; Vc, V. campbellii ; Vv, V. vulnificus; Va, V. alginolyticus

Article Snippet: 1 , ScrR , AGV19641.1 , Sucrose operon repressor , , , V. alginolyticus ATCC 17749 , , .

Techniques: Sequencing

Journal: Journal of pharmacological and toxicological methods

Article Title: Simulated Traumatic Brain Injury in In-Vitro Mouse Neuronal and Brain Endothelial Cell Culture Models

doi: 10.1016/j.vascn.2022.107159

Figure Lengend Snippet: List of Used Material

Article Snippet: Mouse Neuronal Cell Culture Celprogen 11003-02 One primary brain cell line we used in the protocol Mouse Neuronal Cell Culture Complete Growth Glucose and Serum Free Media Celprogen M11003-02GF Flushed with hypoxic gas for hypoxic media preparation (MNCC) Mouse Neuronal Cell Culture Complete Growth Media with 10% Fetal Bovine Serum (FBS) Celprogen M11003-02S For maintenance of MNCC, used for normoxic conditions; also referred to as regular/normal media Mouse Neuronal Cell Culture Extracellular Matrix – 96 Black Wall Well Plates (5/pk) Celprogen E1 1003-02-96BW For fluorometric-based assays (cf.

Techniques: Sparging, Cell Culture, Sterility, Cell Counting, Proliferation Assay, End Point Assay, Activity Assay, CyQUANT Assay, Transferring, Light Microscopy, Saline, LDH Cytotoxicity Assay, Membrane

Journal: iScience

Article Title: Adipocyte-released adipomes in Chagas cardiomyopathy: Impact on cardiac metabolic and immune regulation

doi: 10.1016/j.isci.2024.109672

Figure Lengend Snippet: Adipomes play a major regulatory role in cardiomyocytes functioning (A–C) Fold change in mRNA transcript levels of genes involved in adipogenic and lipogenic signaling (A), mitochondrial oxidative phosphorylation (B), and inflammatory signaling (C) (normalized to Gapdh ) in murine primary cardiomyocytes treated with CLA, ILA, CSA, or ISA for 48 h and compared to untreated cardiomyocytes as demonstrated by qPCR analysis ( n = 3). All adipome-treated groups (CLA, ILA, CSA, and ISA) were compared to untreated groups. Data are represented as mean ± SEM. (∗ p < 0.05, ∗∗ p ≤ 0.01, and ∗∗∗ p ≤ 0.001).

Article Snippet: Adult Mouse Cardiomyocytes were purchased from Celprogen and cultured as a monolayer in flasks pre-coated with Adult Mouse Cardiomyocyte Cell Culture Extra-cellular Matrix.

Techniques:

Journal: iScience

Article Title: Adipocyte-released adipomes in Chagas cardiomyopathy: Impact on cardiac metabolic and immune regulation

doi: 10.1016/j.isci.2024.109672

Figure Lengend Snippet: Circulatory adipomes induce ER stress in cardiomyocytes Immunoblot analysis of β1-AR and CHOP expression in murine primary cardiomyocytes treated with plasma-derived adipomes (P-CLA, P-ILA, P-CSA, and P-ISA) and naive cells ( n = 3/group). Bar graph values were derived from densitometry analysis and by normalizing target protein expression to GDI. The “&” symbol denotes significance calculated between different adipome-treated groups. Data are represented as mean ± SEM. (∗ p < 0.05, ∗∗ p ≤ 0.01, and ∗∗∗ p ≤ 0.001).

Article Snippet: Adult Mouse Cardiomyocytes were purchased from Celprogen and cultured as a monolayer in flasks pre-coated with Adult Mouse Cardiomyocyte Cell Culture Extra-cellular Matrix.

Techniques: Western Blot, Expressing, Derivative Assay

Journal: iScience

Article Title: Adipocyte-released adipomes in Chagas cardiomyopathy: Impact on cardiac metabolic and immune regulation

doi: 10.1016/j.isci.2024.109672

Figure Lengend Snippet: P-ILA cause mitochondrial dysfunction in primary cardiomyocytes (A) Seahorse Mito Stress assay showing the oxygen consumption rate (OCR) from plasma-adipome treated (P-CLA and P-ILA) and untreated mouse cardiomyocytes ( n ≥ 6). Data normalized to nuclear content by staining and fluorescence cell counting. (B–F) Representative graphs depicting the different mitochondrial respiration parameters evaluated, such as basal respiration (B), maximal respiration (C), proton leak (D), spare respiratory capacity (E), and ATP production (F). Data are represented as mean ± SEM. (∗ p < 0.05, ∗∗ p ≤ 0.01, ∗∗∗ p ≤ 0.001, and ∗∗∗ p ≤ 0.0001).

Article Snippet: Adult Mouse Cardiomyocytes were purchased from Celprogen and cultured as a monolayer in flasks pre-coated with Adult Mouse Cardiomyocyte Cell Culture Extra-cellular Matrix.

Techniques: Staining, Fluorescence, Cell Counting

Journal: iScience

Article Title: Adipocyte-released adipomes in Chagas cardiomyopathy: Impact on cardiac metabolic and immune regulation

doi: 10.1016/j.isci.2024.109672

Figure Lengend Snippet:

Article Snippet: Adult Mouse Cardiomyocytes were purchased from Celprogen and cultured as a monolayer in flasks pre-coated with Adult Mouse Cardiomyocyte Cell Culture Extra-cellular Matrix.

Techniques: Infection, Recombinant, Electron Microscopy, SYBR Green Assay, Lysis, Protease Inhibitor, Plasmid Preparation, XF Assay, Isolation, Quantitation Assay, Bicinchoninic Acid Protein Assay, Software